Each project is unique, but below is a general outline of the process for NMR spectroscopy through the MIC:
1 — Contact the MIC to discuss the feasibility of your project. If project is suitable for NMR trials, proceed as below. Suitability of sample for NMR depends on size of protein.
2 — Client completes Sample Information Form and an Authorization for Services and Payment Form.
3 — Client provides MIC with ~10 mg of pure protein sample, at a concentration of ~10 mg/ml, in low ionic strength buffer near neutral pH, for initial feasibility studies.
4 — MIC personnel will transfer sample to NMR tubes for NMR spectral test, and have the spectral data evaluated to determine suitability of protein for further NMR studies. Client must arrange for time & machine with the MU NMR Core Facility.
5 — If feasibility trials indicate your protein is a candidate for further NMR studies (structure, dynamics, molecular recognition, etc.), client should approach Dr. Steve Van Doren or the MU NMR Core Facility to discuss the possibility of a formal collaboration. These collaborations are at the discretion of the above faculty, and would be expected to lead to joint publications and grant funding.
If you have any questions, please contact Dr. Ritcha Mehra-Chaudhary by email at ChaudharyR@missouri.edu or at 573-884-1281.